Rules FAQ
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Highgarden Treasury can only react to cards that explicitly “remove” a character from a challenge via their text. Any other game rules that indirectly cause a character to no longer be participating in a challenge will not trigger Highgarden Treasury. (Source.)
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If an ability removes multiple characters from a challenge at the same time (for instance, A Wall of Roses), each removed character provides its own triggering condition for Highgarden Treasury.