Rules FAQ
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You cannot marshal or ambush an opponent's unique character if you or that opponent already control a copy of that card in play, or if there is a copy of it in either player's dead pile. (See Unique cards in play and in the dead pile.)
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If Barring the Gates is revealed, ambush cannot be used. But you can still marshal cards from any opponent's discard pile during the marshaling phase.
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Cards like The Kingsroad or The Many-Faced God that reduce the cost to marshal or ambush a character still work.